m1 sinobiological 40904 v07h  (Sino Biological)

Journal: medRxiv

Article Title: A three-dose MVA-BN mpox vaccination series improves the quality of anti-monkeypox virus immunity

doi: 10.1101/2025.10.31.25339252

Figure Lengend Snippet: A) Study schedule with median visit times. Ranges of visit times included in each visit are in parentheses. All times are in relation to the previous vaccine dose. Image generated with BioRender. B) Neutralizing titers against clade IIb MPXV as measured by microneutralization assay with 5% guinea pig serum. Mpox convalescent samples are from the indicated time point post-symptom onset. Naïve and experienced controls were collected pre-2022. Black bars represent the geometric mean. Samples with ID 50 values below the limit of detection (LOD) are assigned a value of 10, or ½ the LOD. C) Spearman correlation of IgG for the indicated protein versus MPXV neutralizing titers. Samples are from V4. D) Avidity index for IgG that correlated with MPXV neutralization in both naïve and experienced vaccinees in C. Only avidity measurements from samples with above background binding are included. Black bars represent the mean. Statistics for panel B were performed using the Kruskal-Wallis test with Dunn’s method for multiple comparisons and for panel D were performed using ordinary one-way ANOVA with Šídák’s multiple comparisons test. MPXV Conv, mpox convalescent patients; IMV, intracellular mature virion; EEV, extracellular enveloped virion.

Article Snippet: Briefly, ten different OPXV proteins were included in the panel: MPXV H3 (Sino Biological 40893-V08H1), MPXV A35 (Sino Biological 40886-V08H), MPXV A29 (Sino Biological 40891-V08E), MPXV A30 (Cell Sciences YVV15001A), MPXV M1 (Sino Biological 40904-V07H), MPXV B2 (Abbexa abx620100), MPXV A27 (Abbexa abx620105), MPXV B6 (Abbexa abx620118), VACV A33 (Sino Biological 40896-V07E), and VACV B5 (Sino Biological 40900-V08H).

Techniques: Generated, Microneutralization Assay, Neutralization, Binding Assay

Journal: medRxiv

Article Title: A three-dose MVA-BN mpox vaccination series improves the quality of anti-monkeypox virus immunity

doi: 10.1101/2025.10.31.25339252

Figure Lengend Snippet: A-D) Comparison of MPXV neutralizing titer as assayed in of Robust versus Standard Responders for V3 (A), V4 (B), V5 (C), and V6 (D). E) Comparison of IgG avidity for Robust versus Standard Responders. F) Comparison of dosing interval for Robust versus Standard Responders. Black bars on all panels represent the mean. Statistics for panels A through D and F were performed using the Mann-Whitney test and for panel E were performed using ordinary one-way ANOVA with Šídák’s multiple comparisons test.

Article Snippet: Briefly, ten different OPXV proteins were included in the panel: MPXV H3 (Sino Biological 40893-V08H1), MPXV A35 (Sino Biological 40886-V08H), MPXV A29 (Sino Biological 40891-V08E), MPXV A30 (Cell Sciences YVV15001A), MPXV M1 (Sino Biological 40904-V07H), MPXV B2 (Abbexa abx620100), MPXV A27 (Abbexa abx620105), MPXV B6 (Abbexa abx620118), VACV A33 (Sino Biological 40896-V07E), and VACV B5 (Sino Biological 40900-V08H).

Techniques: Comparison, MANN-WHITNEY

Journal: medRxiv

Article Title: A three-dose MVA-BN mpox vaccination series improves the quality of anti-monkeypox virus immunity

doi: 10.1101/2025.10.31.25339252

Figure Lengend Snippet: A) MPXV neutralization as assayed in for booster subjects in . B) Comparison of peak neutralizing titers following dose two and three. C-G) Avidity index for IgG against MPXV H3 (C), MPXV M1 (D), VACV B5 (E), MPXV A35 (F), and VACV A33 (G). Avidity measurements for post-boost samples use the 1 month post-boost time point with the exception of the measurement for Booster 2 on MPXV A35, which uses the 1 week post-boost time point due to negative levels of anti-MPXV A35 binding at the 1 month time point. Measurements from are shown here for the two dose group. Black bars on panels C through G represent the mean. Statistics were performed using Wilcoxon matched-pairs signed rank test for panel B and ordinary one-way ANOVA with Holm-Šídák’s multiple comparisons test for panels C through G.

Article Snippet: Briefly, ten different OPXV proteins were included in the panel: MPXV H3 (Sino Biological 40893-V08H1), MPXV A35 (Sino Biological 40886-V08H), MPXV A29 (Sino Biological 40891-V08E), MPXV A30 (Cell Sciences YVV15001A), MPXV M1 (Sino Biological 40904-V07H), MPXV B2 (Abbexa abx620100), MPXV A27 (Abbexa abx620105), MPXV B6 (Abbexa abx620118), VACV A33 (Sino Biological 40896-V07E), and VACV B5 (Sino Biological 40900-V08H).

Techniques: Neutralization, Comparison, Binding Assay

Journal: bioRxiv

Article Title: Optimized CRISPR Perturbation Illuminates the Functions of RNA Modification Proteins

doi: 10.1101/2025.07.24.666689

Figure Lengend Snippet: iMAP-91 characterization (A) Atrophic testes. (B) Frequency distribution of the fold-changes for the 79 targeting (pink) and eight NC (cyan) guides across 46 tissues. Black lines mark 2-fold changes, whereas the green lines indicate the 5 th and 95 th percentiles of gNC values. (C) Guide enrichment. Tissue code 01, 02, 03 refers to eyeball, cerebellar central part and cerebellar flocculus, respectively, as specified in (D). Values are mean ± SD. *p < 0.05, **p < 0.01, ***p< 0.001, Student’s t-test. (D) Cluster analysis of guide depletion. Displayed are 87 guides comprising all the guides targeting the 71 RNA modification factors together with all the gNC except the inadvertently duplicated one. Essentiality of the 71 genes per literature is indicated at the top of the heatmap, where shRNA-brain and CRISPR-brain refer to, respectively, the shRNA- and sgRNA-based genomewide in situ screens for neuronal essential genes in the mouse striatum , CRISPR-liver is a genomewide in situ screen for liver essential genes , and CEG2 the core essential gene set identified in human tumor cell lines . CEG2 guides are colored pink. The data points are averaged from 2 to 6 (mostly 5-6) biological replicates.Source data in Table S3. (E) Editing rates of representative gRNAs selected from iMAP-91. gRNAs and Cas9 were co-expressed in N2a cells in duplicates before indel quantification. (F) Cascade reaction leading to tRNA-U34 modifications (Rapino et al., 2017b). (G) Guide clustering coincides with the phenotypes of global single-gene KO mice. Analyzed are the genes whose KO phenotypes are available from MGI. The number of genes in each category is indicated. Source data in Table S3 (sheet 2).

Article Snippet: RNP was assembled by incubating 40 pm each of recombinant Cas9 (Sino Biological) and sgRNA or non-targeting control (Table S1 sheet 3) at 37°C for 15 min. RNP was mixed with 3×106 NK cells resuspended in 20 μl P3 Primary Cell Solution (Lonza) before electroporation using a 4D-NucleofectorTM (program CM-137).

Techniques: RNA modification, shRNA, CRISPR, In Situ